Catechins from Green tea (Camellia sinensis) is widely known for
many biological activities, special anti-cancer and anti-inflammatory properties.
Catechins were extracted by mechanical blending, and using citric
acid, with 2.31% in extraction efficiency. The result of determining by
HPLC-UV showed that Catechin< EC < EGC < ECG < EGCG in
concentration compared to dry powder, reaching a total content of 634.51
mg/g, higher than tea powder (537.65 mg/g). Then, anti-inflammatory
and anticancer activities of these extracts were investigated. Cell viability
assay (MTT) showed that catechin extracts were not toxic to Normal Human
Dermal Fibroblasts Cells. They even exhibited anti-inflammatory activities by suppressing the production of NO and proinflammatory cytokines.
The results also showed that the extracted catechin powder
also showed good anti-inflammatory activity, at a concentration of 45
?g/mL, NO proliferation reached 44.4%. Anticancer activities of these
extracts were displayed through their ability to inhibit the growth of cancer
cell lines: liver cancer cell line (HepG2), lung cancer cell line (A549),
leukemia cancer cell line (K562), with IC50 values of 181.1 μg/mL, 186
μg/mL, 137.8 μg/mL, respectively. So catechin extracts from green tea
may have the potential to be applied in pharmaceutical.