Journal Articles - Natural Science - 2019

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  • Publication
    Effect of different Sophora flavescens extracts on proliferation of HepG2 and BT474 cell lines
    ( 2019)
    Cao Ngoc Minh Trang
    ;
    Le Thanh Long
    ;
    Ngo Dai Nghiep
    ;
    Hoang Nghia Son
    This study aimed to estimate the changes of proliferation of HepG2 and BT474 cell lines under the treatment with Sophora flavescens extracts by Ethyl acetate and Chloroform methods. The cell proliferation was evaluated by WST-1 assay. The HepG2 and BT474 cell lines were treated with Ethyl acetate extract and Chloroform extract at a concentration of 3.125, 6.25, 12.5, 25, 50, and 100 μg/ml. The results showed that the proliferation of HepG2 cell was reduced by treatment of 25 μg/ml Ethyl acetate extract. The reduced proliferation was observed in HepG2 by treatment with 25 μg/ml Chloroform extract. The Chloroform extract also induced the decrease of proliferation in BT474 cell line while there was no statistically significant difference in BT474 cell proliferation between groups by the treatment of Ethyl acetate extract. The Chloroform extract and Ethyl acetate extracts gave IC50 values of 47.08 and 45.92 μg/ml against the Hep G2 cell line. This result suggested that Chloroform extract and Ethyl acetate extracts of Sophora flavescens were efficient to inhibition of HepG2 proliferation.
  • Publication
    Genetic relationships of the Anabas (ANABAS SP.) using mitochondrial cytochrome oxidase subunit I marker gene
    ( 2019)
    Trương Thế Quang
    Total DNA of the anabas specimens (A1, A2, A3, A4) in Vietnam were extracted using PHUSA-IHHNV kit and according to the process of Phu Sa Biochemical Company. The mitochondrial cytochrome oxidase subunit I (COI) gene were amplified using PCR reaction. The obtained PCR products were checked using 2 % agarose gel electrophoresis and were sequenced with both the forward and reverse primers using an automated sequencer ABI 3730XL of Applied Biosystems by Sangers method. The genetic distances (Dij) between the anabas species in Vietnam (A1, A2, A3, A4) are very small from 0.0046 to 0.0156 and they are all in group 4 with Anabas testudineus in Malaysia so they are the same Anabas testudineus. The researched species of Anabas genus in Vietnam (A1, A2, A3, A4) and some countries in South and Southeast Asia were divided into four groups based on COI marker gene. The groups with far genetic distance include group 2 and group 4 (D24 = 0.1055), group 1 and group 4 (D14 = 0.1041), group 1 and group 3 (D13 = 0.0910). The groups with medium genetic distance include group 2 and group 3 (D23 = 0.0849), group 1 and group 2 (D12 = 0.0772). The groups with near genetic distance include group 3 and group 4 (D34 = 0.0285). The species Anabas testudineus in India and Anabas testudineus in Myanmar should be re-identified Anabas cobojius in India and Anabas cobojius in Myanmar belong to group 1.
  • Publication
    New diterpenoids from the stems of Euphorbia antiquorum growing in Vietnam
    ( 2020)
    Thanh-Nha Tran
    ;
    Jirapast Sichaem
    ;
    Van-Kieu Nguyen
    ;
    Huu-Hung Nguyen
    ;
    Truong-Tam Cao
    ;
    Thi-Phuong Nguyen
    ;
    Van-Giau Vo
    ;
    Nakorn Niamnont
    ;
    Ngoc-Hong Nguyen
    ;
    Thuc-Huy Duong
    Phytochemical analysis of Euphorbia antiquorum stem extracts afforded two new ent-atisane compounds, ent-3α-acetoxy-16β,17,18-trihydroxyatisane (1) and ent-3α,14,16β,17-tetrahydroxyatisane (2) together with three known compounds, 20-deoxy-16-hydroxyingenol (3), ent-14[S],16α,17- trihydroxyatisan-3-one (4), and agallochaol C (5). Their structures were elucidated by spectroscopic data analysis and comparison with published NMR data. Compounds 1-5 were evaluated for α-glucosidase inhibition and cytotoxicity. Compounds 1, 4, and 5 revealed significant inhibitory activity against α-glucosidase with the IC50 values of 119.9, 135.5, and 134.3 μM, respectively. None showed activity in cytotoxicity assay.
  • Publication
    Study on the Ability of extracts from Cordyceps Spp. Biomass to prevent long term memory impairment in mice by morris water maze
    ( 2016)
    Dang Hoang Quyen
    ;
    Tran Thi Hoang Yen
    ;
    Vo Thi Xuyen
    ;
    Dinh Minh Hiep
    ;
    Truong Binh Nguyen
    The ability of three mycelial extracts of Cordyceps spp. (Poly DL0004, n-BuOH DL0015 and n-BuOH DL0006) to prevent long-time memory impairment of mice were tested by using Morris water maze model. Mice were induced memory impairment by Trymethyltin (TMT) as dose of 2.4 mg/kg (i.p. injection); then fed by solution of Cordyceps's mycelial extract as doses of 100 mg/kg (p.o) and 200 mg/kg (p.o); and test in Morris water maze model. The behavioral test of those mice was illustrated by concentration of neurotransmitter Acetylcholine (ACh) and its degraded enzyme Acetylcholinesterase (AChE) in their hippocampus. As results, Poly DL0004 and n-BuOH DL0015 had best effects on memory improvement at doses of 100 mg/kg in hidden platform test and probe test but no effect on working memory test. The concentrations of ACh and AChE in mice hippocampus were significant different between mice fed by Cordyceps's mycelial extract and negative control mice injected TMT dose of 2.4 mg/kg. No difference of concentrations of ACh and AChE were recorded between mice fed by extracts dose of 100 mg/kg and 200 mg/kg. The memory improvement effects of Cordyceps's mycelial extract were the first step to potentially apply Cordyceps products for human healthcare in Vietnam.