Journal Articles - Engineering Technology - 2022
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PublicationPhyto-fabrication of silver nanoparticle using leaf extracts of Aristolochia bracteolata Lam and their mosquito larvicidal potential( 2022)This research was designed to evaluate the fine quality of silver nanoparticles (AgNPs) fabricating proficiency of aqueous leaf extract of Aristolochia bracteolata and evaluate their larvicidal potential against I-IVth instar larvae and pupa of Culex quinquefasciatus. Within 24 h, the A. bracteolata aqueous leaf extract significantly reduced the AgNO3 and yielded AgNPs. It was initially confirmed by a visible color change from pale yellow to dark reddish brown in reaction mix and under UV–vis spectrum analysis the absorbance peak related to AgNPs was found at 430 nm. The nature of the phyto-synthesized AgNPs was characterized by standard analytical techniques such as Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Energy Dispersive X-Ray analyses (EDX). The A. bracteolata synthesized AgNPs were predominantly spherical in shape and size ranging from 29.30 to 46.32 nm. The percentage of Ag elemental composition was found as 57.61 % using EDX analysis. The A. bracteolata yielded AgNPs showed significant larvicidal and pupicidal efficiency on youngster (I-IVth instar larvae and pupa) of Culex quinquefasciatus mosquito vector. These results conclude that the aqueous leaf extract of A. bracteolata can produce biologically active AgNPs.
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PublicationPrediction of the fuel spray characteristics in the combustion chamber with methane and TiO2 nanoparticles via numerical modelling( 2022)In this study the methane combustion was analysed with the TiO2 nanoparticles. A series of the simulation runs were performed by varying the fuel inlet velocity. However, the oxidizer and the nanoparticles spray were maintained constant for the entire run. The spray velocity varied from 100 m/s to 200 m/s with titanium dioxide (TiO2) nanoparticles. Using the series of the governing equation and modified Navier Stokes equation the model has been developed with the aid of numerical workbench. Three different domains are generated for fuel, oxidizer and nanoparticles. The velocity of the air and nanoparticles were maintained at constant levels and varying only the spray velocity of the fuel. Based on the findings, the mass fraction of both fuel and formation of the CO2 were dependent on the spray velocity. As the spray velocity increases the turbulence in the combustion chamber increases which ensures the higher mixing of both air–fuel and nanoparticles. From the procured findings 175 m/s and 200 m/s were the ideal range for better combustion efficiency compared to 100 m/s and 150 m/s. The simulation results have ascertained the role of the spray velocity on the emissions and the combustion efficiency of the engine. It is hoped that obtained results can provide directions to work on the combustion of the methane with the nanoparticles at the optimized spray velocity.
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PublicationSynthesis of copper nanoparticles from the aqueous extract of Cynodon dactylon and evaluation of its antimicrobial and photocatalytic properties( 2022)The copper nanoparticles (CuNPs) synthesizing potential of Cynodon dactylon aqueous leaf extract and their antibacterial as well as dye degradation potentials were investigated. The synthesized CuNPs was initially characterized by gradual colour change from dark brown to blue in colour and then found absorbance peak at 469 nm. Furthermore, the SEM and DLS analyses showed that biosynthesized CuNPs were spherical in shaped and size ranging from 120 to 129 nm. The FTIR spectrum confirmed the presence of flavonoids, alkaloids, terpenoids, and phenols, which involved in the reduction, capping, and stabilization of CuNPs. This green synthesized CuNPs also demonstrated remarkable antibacterial activity against the bacterial pathogens such as Escherichia coli, Bacillus subtilis and Staphylococcus aureus and Klebsiella pneumoniae. This green synthesized CuNPs exhibited considerable dye degrading potential in the following order as methyl organge > methyl red > Erichrome black T dyes in the presence of sunlight through photocatalytic degradation process. These results conclude that C. dactylon aqueous leaf extract mediated nanoparticles possess remarkable antibacterial and dye degrading potential.
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PublicationPhytoremediation potential of Gossypium hirsutum on abandoned polluted chromium sludge soil with the amalgamation of Streptomyces tritici D5( 2022)The phytoremediation potency of Gossypium hirsutum was explored in this research under the influence of preidentified metal tolerant Streptomyces tritici D5 in Cr enriched sludge soil using various treatment sets (I to V) in a greenhouse setting. Interestingly, the G. hirsutum remarkable remediate the Cr metal from the Cr enriched sludge soil under diluted (50:50) condition in 90 days of greenhouse experiment. The S. tritici D5 also effectively support the growth and phytoremediation competence of G. hirsutum. This was evidenced by the under the diluted (set III) condition the growth and major biomolecules such as protein, carbohydrate, and chlorophyll content of G. hirsutum were considerably increased in quantity. Hence, the phytoremediation potential of G. hirsutum was effective at soil diluted with fertile and xenobiotics free soil with dilution ratio of 50:50 (set III) and followed by 75:25 (set II) ratio. Thus, under diluted conditions (50:50) G. hirsutum seed coated with S. tritici D5 showed an outstanding phytoremediation process. Therefore, this method can be implemented to the field level study to assess the metal removal prospects of this environmentally friendly method.
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PublicationSustainable bioremediation approach to treat the sago industry effluents and evaluate the possibility of yielded biomass as a single cell protein (SCP) using cyanide tolerant Streptomyces tritici D5( 2022)This sustainable approach was performed to evaluate the bioremediation potential of cyanide resistant bacterial species on sago industry effluents and assess the possibility of using the yielded biomass as single cell protein (SCP). The predominant cyanide tolerant bacterium enumerated from muddy soil was identified as Streptomyces tritici D5 through 16S rRNA sequencing. The identified S. tritici D5 strains showed excellent resistant and degradation potential at 100 mM concentration of potassium cyanide. Furthermore, the physicochemical properties analysis of sago industry effluents results revealed that the most of the parameters were crossing the permissible limits of Pollution control board of India. The bioremediation process was performed at various temperatures at 25 ◦C, 35 ◦C, and 45 ◦C for a period of 30 days of continuous bioremediation process with the aid of an aerator. Surprisingly, the best organic pollutant reduction was found at 35 ◦C and 45 ◦C, with 25 ◦C following close behind. Remarkably, the dissolved oxygen (DO) level was gradually increased from 2.24 to 12.04 mg L 1 at 35 ◦C in 30 days of the remediation process. The pH and ammonia were also significantly increased during the bioremediation process in 30 days of treatment. Similarly, at 35 ◦C of bioremediation process the S. tritici D5 yielded maximum dried biomass (6.9 g L 1) with the total crude protein (SCP) as 4.8 g L 1 (69.56%) in 30 days of growth. These findings stated that S. tritici D5 can treat sago industry effluents and that the biomass produced may be considered SCP after some in-vitro and in-vivo analyses.