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Silk fibroin hydrogel containing <i>Sesbania sesban</i> L. extract for rheumatoid arthritis treatment
Silk fibroin hydrogel containing <i>Sesbania sesban</i> L. extract for rheumatoid arthritis treatment
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Date
2022
Authors
Duy Toan Pham
Nguyen Thi Phuong Thao
Bui Thi Phuong Thuy
Van De Tran
Thanh Q. C. Nguyen
Ngoc Nha Thao Nguyen
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Research Projects
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Abstract
Purpose: Rheumatoid arthritis, a chronic and progressive inflammation condition in the joints, has significantly
reduced the patient quality of life and life expectancy. Crucially, there is no complete therapy
for this disease, and the current treatments possess numerous side effects. Thus, novel
therapeutic approach is necessary. To that end, this study developed novel silk fibroin in-situ hydrogel
containing Sesbania sesban L. extract, a plant with high anti-inflammatory actions that are beneficial
for rheumatoid arthritis treatments.
Methods: The hydrogels were manufactured using simple method of spontaneous gelation at different
temperature. The gel properties of morphology, gelation time, viscosity, gel strength, stability,
drug loading capacity, drug release rate, and in-vitro anti-inflammatory activity were investigated with
appropriate methods.
Results: The optimal formulation had highly porous structure, with a gelation time of 0.5 h at room
temperature and bodily temperature of 37 C, a viscosity of 2530 ± 50 cP, a gel strength of
1880.14 ± 35.10 g, and a physical stability of >6months. Moreover, the hydrogel contained the
Sesbania sesban L. leaf extract with a total phenolic content of 92.8 ± 8.30mg GAE/g, and sustained
the release rate for >20 da: ys, followed the Higuchi model. Regarding the in-vitro activities, all formulations
were nontoxic to the RAW 264.7 cell line and demonstrated comparable anti-inflammatory
activity to the free extract, in terms of the NO reduction levels.
Conclusion: Conclusively, the systems possessed potential properties to be further investigated to
become a prospective rheumatoid arthritis treatment.
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Keywords
"Silk fibroin,
Hydrogel,
Sesbania sesban,
rheumatoid arthritis,
antiinflammation"