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Purification of Alpha fetoprotein from Human Umbilical Cord Plasma

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datacite.subject.fos oecd::Natural sciences
dc.contributor.author Nguyễn Hữu Hùng
dc.date.accessioned 2022-11-19T03:08:06Z
dc.date.available 2022-11-19T03:08:06Z
dc.date.issued 2021
dc.description.abstract Alpha-fetoprotein (AFP) is a sera protein that involves in fetal development, produced by the fetal liver and yolk sac. Human AFP (hAFP) is not detected in adults but it is increased in cases of hepatocellular carcinoma (HCC), cirrhosis and other cancers like germ cell cancer, stomach and intestine cancer. hAFP has been used as an important marker for HCC serological diagnosis and it has recently become a target for HCC immunization studies. Umbilical cord blood has approximately 150–250 μg/ml hAFP but it is usually discarded in hospitals. In order to take advantage of human umbilical cord blood as hAFP nature material, this study have established a three-step chromatography process for hAFP purification including removal of albumin by Hitrap blue HP column, hAFP captured chromatography using rabbit anti-hAFP IgG column, and filtration chromatography through Sephacryl S100 HR column. By native-PAGE, western blotting and bradford assays, the hAFP purity was showed more than 95% and 80 μg of hAFP per mL of initial cord plasma was recovered. Our established hAFP purification process can be performed on a larger scale for later applications in the serodiagnosis of HCC and other hAFP related diseases.
dc.identifier.doi 10.1007/978-3-030-75506-5_41
dc.identifier.isbn 9783030755058
dc.identifier.isbn 9783030755065
dc.identifier.uri http://repository.vlu.edu.vn:443/handle/123456789/1445
dc.language.iso en_US
dc.relation.ispartof IFMBE Proceedings
dc.relation.ispartof 8th International Conference on the Development of Biomedical Engineering in Vietnam
dc.relation.issn 1680-0737
dc.relation.issn 1433-9277
dc.subject Alpha-fetoprotein
dc.subject Chromatography
dc.subject Hepatocellular carcinoma
dc.subject Purification
dc.subject Umbilical cord blood
dc.title Purification of Alpha fetoprotein from Human Umbilical Cord Plasma
dc.type Resource Types::text::conference output::conference proceedings
dspace.entity.type Publication
oairecerif.author.affiliation #PLACEHOLDER_PARENT_METADATA_VALUE#
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