Publication:
Effect of different Sophora flavescens extracts on proliferation of HepG2 and BT474 cell lines
Effect of different Sophora flavescens extracts on proliferation of HepG2 and BT474 cell lines
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Date
2019
Authors
Cao Ngoc Minh Trang
Le Thanh Long
Ngo Dai Nghiep
Hoang Nghia Son
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Abstract
This study aimed to estimate the changes of proliferation of HepG2 and BT474 cell lines under the
treatment with Sophora flavescens extracts by Ethyl acetate and Chloroform methods. The cell
proliferation was evaluated by WST-1 assay. The HepG2 and BT474 cell lines were treated with
Ethyl acetate extract and Chloroform extract at a concentration of 3.125, 6.25, 12.5, 25, 50, and 100
μg/ml. The results showed that the proliferation of HepG2 cell was reduced by treatment of 25 μg/ml
Ethyl acetate extract. The reduced proliferation was observed in HepG2 by treatment with 25 μg/ml
Chloroform extract. The Chloroform extract also induced the decrease of proliferation in BT474 cell
line while there was no statistically significant difference in BT474 cell proliferation between groups
by the treatment of Ethyl acetate extract. The Chloroform extract and Ethyl acetate extracts gave
IC50 values of 47.08 and 45.92 μg/ml against the Hep G2 cell line. This result suggested that
Chloroform extract and Ethyl acetate extracts of Sophora flavescens were efficient to inhibition of
HepG2 proliferation.
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Keywords
"HepG2 cell line,
BT474 cell line,
proliferation,
Sophora flavescens extract"