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Evaluation of the Anti Inflammatory and Anticancer Activities of Catechins Extracted from Green Tea (Camellia Sinensis) in Some Cancer Cell Lines
Evaluation of the Anti Inflammatory and Anticancer Activities of Catechins Extracted from Green Tea (Camellia Sinensis) in Some Cancer Cell Lines
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Date
2020
Authors
Ho Thi Ngoc Tram
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Abstract
Catechins from Green tea (Camellia sinensis) is widely known for
many biological activities, special anti-cancer and anti-inflammatory properties.
Catechins were extracted by mechanical blending, and using citric
acid, with 2.31% in extraction efficiency. The result of determining by
HPLC-UV showed that Catechin< EC < EGC < ECG < EGCG in
concentration compared to dry powder, reaching a total content of 634.51
mg/g, higher than tea powder (537.65 mg/g). Then, anti-inflammatory
and anticancer activities of these extracts were investigated. Cell viability
assay (MTT) showed that catechin extracts were not toxic to Normal Human
Dermal Fibroblasts Cells. They even exhibited anti-inflammatory activities by suppressing the production of NO and proinflammatory cytokines.
The results also showed that the extracted catechin powder
also showed good anti-inflammatory activity, at a concentration of 45
?g/mL, NO proliferation reached 44.4%. Anticancer activities of these
extracts were displayed through their ability to inhibit the growth of cancer
cell lines: liver cancer cell line (HepG2), lung cancer cell line (A549),
leukemia cancer cell line (K562), with IC50 values of 181.1 μg/mL, 186
μg/mL, 137.8 μg/mL, respectively. So catechin extracts from green tea
may have the potential to be applied in pharmaceutical.
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Keywords
Green tea extract,
Camellia sinensis,
catechins,
anti-inflammatory,
anticancer,
HepG2,
A549,
K562