Effect of different Sophora flavescens extracts on proliferation of HepG2 and BT474 cell lines

This study aimed to estimate the changes of proliferation of HepG2 and BT474 cell lines under the treatment with Sophora flavescens extracts by Ethyl acetate and Chloroform methods. The cell proliferation was evaluated by WST-1 assay. The HepG2 and BT474 cell lines were treated with Ethyl acetate extract and Chloroform extract at a concentration of 3.125, 6.25, 12.5, 25, 50, and 100 μg/ml. The results showed that the proliferation of HepG2 cell was reduced by treatment of 25 μg/ml Ethyl acetate extract. The reduced proliferation was observed in HepG2 by treatment with 25 μg/ml Chloroform extract. The Chloroform extract also induced the decrease of proliferation in BT474 cell line while there was no statistically significant difference in BT474 cell proliferation between groups by the treatment of Ethyl acetate extract. The Chloroform extract and Ethyl acetate extracts gave IC50 values of 47.08 and 45.92 μg/ml against the Hep G2 cell line. This result suggested that Chloroform extract and Ethyl acetate extracts of Sophora flavescens were efficient to inhibition of HepG2 proliferation.